Subject(s)
Humans , Female , Amniotic Fluid/microbiology , Herpes Genitalis/microbiology , Immunoglobulins/analysis , Pregnancy Complications, Infectious/microbiology , Simplexvirus/isolation & purification , Adult , Amniotic Fluid/immunology , Antibodies, Viral/analysis , Chile/epidemiology , English Abstract , Follow-Up Studies , Herpes Genitalis/epidemiology , Herpes Genitalis/immunology , Herpes Genitalis/pathology , Pregnancy , Placenta/pathology , Pregnancy Complications, Infectious/epidemiologyABSTRACT
A total of 60 herpes simplex virus (HSV) isolates obtained from individuals with genital herpes lesions in the mouth or respiratory illness were typed in this study. The HSV isolation rate in genital herpes was 31%. The typing was done by a direct fluorescent monoclonal antibody technique using commercial reagents. All the 60 Vero cell grown isolates were successfully typed without any indeterminate results. Nine isolates were typed as HSV 1 and 51 as HSV 2. The technique was easy to perform and read. HSV 2 was found to predominate in genital infections and HSV 1 in respiratory infections.
Subject(s)
Fluorescent Antibody Technique , Herpes Genitalis/microbiology , Herpes Simplex/microbiology , Humans , Respiratory Tract Infections/microbiology , Serotyping , Simplexvirus/classification , Stomatitis, Herpetic/microbiologyABSTRACT
Herpes simplex viruses were isolated from 40.8 to 56.0 per cent of the patients with genital herpes. The frequency of recovery seemed to be higher in females than in males, particularly during the first episode of infection. Asymptomatic shedding of the virus from female genitalias was approximately 0.7 per cent. Herpes simplex virus type 2 represented 98.4 per cent of all isolates and the remaining isolates were type 1. These isolates exhibited a wide range of sensitiveness to 9-(2-hydroxyethoxymethyl) guanine (acyclovir) as demonstrated by antiviral inhibition assay and no single strain exhibited high in vitro drug ID50 value.